A microfluidic immunomagnetic bead-based system for the rapid detection of influenza infections: from purified virus particles to clinical specimens
Identifieur interne : 000874 ( Ncbi/Merge ); précédent : 000873; suivant : 000875A microfluidic immunomagnetic bead-based system for the rapid detection of influenza infections: from purified virus particles to clinical specimens
Auteurs : Lien-Yu Hung ; Tze-Bin Huang ; Yi-Che Tsai ; Chen-Sheng Yeh ; Huan-Yao Lei ; Gwo-Bin LeeSource :
- Biomedical Microdevices [ 1387-2176 ] ; 2013.
Abstract
Seasonal and novel influenza infections have the potential to cause worldwide pandemics. In order to properly treat infected patients and to limit its spread, a rapid, accurate and automatic influenza diagnostic tool needs to be developed. This study therefore presents a new integrated microfluidic system for the rapid detection of influenza infections. It integrated a suction-type, pneumatic-driven microfluidic control module, a magnetic bead-based fluorescent immunoassay (FIA) and an end-point optical detection module. This new system can successfully distinguish between influenza A and B using a single chip test within 15 min automatically, which is faster than existing devices. By utilizing the micromixers to thoroughly wash out the sputum-like mucus, this microfluidic system could be used for the diagnosis of clinical specimens and reduced the required sample volume to 40 μL. Furthermore, the results of diagnostic assays from 86 patient specimens have demonstrated that this system has 84.8 % sensitivity and 75.0 % specificity. This developed system may provide a powerful platform for the fast screening of influenza infections.
The online version of this article (doi:10.1007/s10544-013-9753-0) contains supplementary material, which is available to authorized users.
Url:
DOI: 10.1007/s10544-013-9753-0
PubMed: 23420191
PubMed Central: 7087985
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In order to properly treat infected patients and to limit its spread, a rapid, accurate and automatic influenza diagnostic tool needs to be developed. This study therefore presents a new integrated microfluidic system for the rapid detection of influenza infections. It integrated a suction-type, pneumatic-driven microfluidic control module, a magnetic bead-based fluorescent immunoassay (FIA) and an end-point optical detection module. This new system can successfully distinguish between influenza A and B using a single chip test within 15 min automatically, which is faster than existing devices. By utilizing the micromixers to thoroughly wash out the sputum-like mucus, this microfluidic system could be used for the diagnosis of clinical specimens and reduced the required sample volume to 40 μL. Furthermore, the results of diagnostic assays from 86 patient specimens have demonstrated that this system has 84.8 % sensitivity and 75.0 % specificity. This developed system may provide a powerful platform for the fast screening of influenza infections.</p><sec><title>Electronic supplementary material</title><p>The online version of this article (doi:10.1007/s10544-013-9753-0) contains supplementary material, which is available to authorized users.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Atmar, Rl" uniqKey="Atmar R">RL Atmar</name></author><author><name sortKey="Baxter, Bd" uniqKey="Baxter B">BD Baxter</name></author><author><name sortKey="Dominguez, Ea" uniqKey="Dominguez E">EA Dominguez</name></author><author><name sortKey="Taber, Lh" uniqKey="Taber L">LH Taber</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Both, Gw" uniqKey="Both G">GW Both</name></author><author><name sortKey="Sleigh, Mj" uniqKey="Sleigh M">MJ Sleigh</name></author><author><name sortKey="Cox, Nj" uniqKey="Cox N">NJ Cox</name></author><author><name 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sortKey="Hsiung, Sk" uniqKey="Hsiung S">SK Hsiung</name></author><author><name sortKey="Lee, Gb" uniqKey="Lee G">GB Lee</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zheng, Hj" uniqKey="Zheng H">HJ Zheng</name></author><author><name sortKey="Tao, Zh" uniqKey="Tao Z">ZH Tao</name></author><author><name sortKey="Cheng, Wf" uniqKey="Cheng W">WF Cheng</name></author><author><name sortKey="Piessens, Wf" uniqKey="Piessens W">WF Piessens</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Biomed Microdevices</journal-id><journal-id journal-id-type="iso-abbrev">Biomed Microdevices</journal-id><journal-title-group><journal-title>Biomedical Microdevices</journal-title></journal-title-group><issn pub-type="ppub">1387-2176</issn><issn pub-type="epub">1572-8781</issn><publisher><publisher-name>Springer US</publisher-name><publisher-loc>Boston</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">23420191</article-id><article-id pub-id-type="pmc">7087985</article-id><article-id pub-id-type="publisher-id">9753</article-id><article-id pub-id-type="doi">10.1007/s10544-013-9753-0</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>A microfluidic immunomagnetic bead-based system for the rapid detection of influenza infections: from purified virus particles to clinical specimens</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Hung</surname><given-names>Lien-Yu</given-names></name><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Huang</surname><given-names>Tze-Bin</given-names></name><xref ref-type="aff" rid="Aff2">2</xref></contrib><contrib contrib-type="author"><name><surname>Tsai</surname><given-names>Yi-Che</given-names></name><xref ref-type="aff" rid="Aff3">3</xref></contrib><contrib contrib-type="author"><name><surname>Yeh</surname><given-names>Chen-Sheng</given-names></name><xref ref-type="aff" rid="Aff4">4</xref></contrib><contrib contrib-type="author"><name><surname>Lei</surname><given-names>Huan-Yao</given-names></name><xref ref-type="aff" rid="Aff3">3</xref></contrib><contrib contrib-type="author" corresp="yes"><name><surname>Lee</surname><given-names>Gwo-Bin</given-names></name><address><phone>+886-3-5715131</phone><fax>+886-3-5722840</fax><email>gwobin@pme.nthu.edu.tw</email></address><xref ref-type="aff" rid="Aff1">1</xref></contrib><aff id="Aff1"><label>1</label><institution-wrap><institution-id institution-id-type="GRID">grid.38348.34</institution-id><institution-id institution-id-type="ISNI">0000000405320580</institution-id><institution>Department of Power Mechanical Engineering,</institution><institution>National Tsing Hua University,</institution></institution-wrap>Hsinchu, Taiwan 30013</aff><aff id="Aff2"><label>2</label><institution-wrap><institution-id institution-id-type="GRID">grid.38348.34</institution-id><institution-id institution-id-type="ISNI">0000000405320580</institution-id><institution>Institute of NanoEngineering and MicroSystems, National Tsing Hua University,</institution></institution-wrap>Hsinchu, Taiwan 30013</aff><aff id="Aff3"><label>3</label><institution-wrap><institution-id institution-id-type="GRID">grid.64523.36</institution-id><institution-id institution-id-type="ISNI">0000000405323255</institution-id><institution>Department of Microbiology and Immunology,</institution><institution>National Cheng Kung University,</institution></institution-wrap>Tainan, Taiwan 70101</aff><aff id="Aff4"><label>4</label><institution-wrap><institution-id institution-id-type="GRID">grid.64523.36</institution-id><institution-id institution-id-type="ISNI">0000000405323255</institution-id><institution>Department of Chemistry,</institution><institution>National Cheng Kung University,</institution></institution-wrap>Tainan, Taiwan 70101</aff></contrib-group><pub-date pub-type="epub"><day>19</day><month>2</month><year>2013</year></pub-date><pub-date pub-type="ppub"><year>2013</year></pub-date><volume>15</volume><issue>3</issue><fpage>539</fpage><lpage>551</lpage><permissions><copyright-statement>© Springer Science+Business Media New York 2013</copyright-statement><license><license-p>This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.</license-p></license></permissions><abstract id="Abs1"><p>Seasonal and novel influenza infections have the potential to cause worldwide pandemics. In order to properly treat infected patients and to limit its spread, a rapid, accurate and automatic influenza diagnostic tool needs to be developed. This study therefore presents a new integrated microfluidic system for the rapid detection of influenza infections. It integrated a suction-type, pneumatic-driven microfluidic control module, a magnetic bead-based fluorescent immunoassay (FIA) and an end-point optical detection module. This new system can successfully distinguish between influenza A and B using a single chip test within 15 min automatically, which is faster than existing devices. By utilizing the micromixers to thoroughly wash out the sputum-like mucus, this microfluidic system could be used for the diagnosis of clinical specimens and reduced the required sample volume to 40 μL. Furthermore, the results of diagnostic assays from 86 patient specimens have demonstrated that this system has 84.8 % sensitivity and 75.0 % specificity. This developed system may provide a powerful platform for the fast screening of influenza infections.</p><sec><title>Electronic supplementary material</title><p>The online version of this article (doi:10.1007/s10544-013-9753-0) contains supplementary material, which is available to authorized users.</p></sec></abstract><kwd-group xml:lang="en"><title>Keywords</title><kwd>Microfluidics</kwd><kwd>Magnetic bead</kwd><kwd>Rapid detection</kwd><kwd>Influenza</kwd><kwd>Clinical specimen</kwd></kwd-group><custom-meta-group><custom-meta><meta-name>issue-copyright-statement</meta-name><meta-value>© Springer Science+Business Media New York 2013</meta-value></custom-meta></custom-meta-group></article-meta></front></pmc><affiliations><list></list><tree><noCountry><name sortKey="Huang, Tze Bin" sort="Huang, Tze Bin" uniqKey="Huang T" first="Tze-Bin" last="Huang">Tze-Bin Huang</name><name sortKey="Hung, Lien Yu" sort="Hung, Lien Yu" uniqKey="Hung L" first="Lien-Yu" last="Hung">Lien-Yu Hung</name><name sortKey="Lee, Gwo Bin" sort="Lee, Gwo Bin" uniqKey="Lee G" first="Gwo-Bin" last="Lee">Gwo-Bin Lee</name><name sortKey="Lei, Huan Yao" sort="Lei, Huan Yao" uniqKey="Lei H" first="Huan-Yao" last="Lei">Huan-Yao Lei</name><name sortKey="Tsai, Yi Che" sort="Tsai, Yi Che" uniqKey="Tsai Y" first="Yi-Che" last="Tsai">Yi-Che Tsai</name><name sortKey="Yeh, Chen Sheng" sort="Yeh, Chen Sheng" uniqKey="Yeh C" first="Chen-Sheng" last="Yeh">Chen-Sheng Yeh</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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